The Sylvester & Tweety Mysteries, produced by Warner Bros. Animation, is an animated television series which aired from 1995 to 2000 on Kids' WB.

Sylvester (voiced by Joe Alaskey) – Sylvester acts as narrator throughout the episodes, and continually tries to eat Tweety, though he is always thwarted by Hector or Tweety's craftiness. Despite their never-ending feud, the pair manage to get along for the most part, and Sylvester will defend Tweety when someone else tries to harm the canary. Sylvester also unwittingly discovers many clues, claiming as the narrator to have knowledge of the case (though this is probably bluster). Besides chasing Tweety, Sylvester often explores the surroundings, leading him to sometimes be in awkward situations (i.e. being chased by a male cousin of Pepe Le Pew or using Granny's umbrella to pierce a villain's blimp). Through these situations, the Tuxedo cat endures all manner of pain and suffering, but comes out none the worse for wear. Sylvester is allergic to wool and is jealous of Tweety.

Tweety (voiced by Joe Alaskey) – A cute Yellow canary. Tweety is often trying to escape Sylvester by foiling him through either his flying, clever timing, or his virtual bodyguard, Hector. Tweety usually stays with Granny and is responsible for some major clues. Despite his feud with Sylvester, the pair will team up if Granny is threatened and can work together very well. Tweety also serves as a source of ironic humor at times, usually in reference to Sylvester's foiled attempts to eat him. The show also harks back to Tweety's earlier shorts, and he is not above malice towards "dat bad old putty tat".

Granny (voiced by June Foray) – A practical old fashioned world-renowned detective, Granny travels the globe with Sylvester and Tweety, attending events such as races or canary contest and is often called upon by the locals to solve a crime. However, there have been attempts to frame Granny, causing some difficulty in finding clues. She is overprotective of Tweety, and will not hesitate to give Sylvester a good whack on the head. She sometimes calls upon the cat to investigate clues or defend the group while they sleep, though, showing signs of obvious trust. Granny is good natured mostly, but can become stern. With Granny as the main detective of the show, she bears similarity to Miss Marple.

Hector (voiced by Frank Welker) – Granny's bulldog and Tweety's bodyguard. Hector keeps Sylvester from eating the canary and will often beat him up if he gets in his way. Hector will defend Granny, Tweety or even Sylvester if threatened, but is not above stealing food or trying to please others.